促销 β-Catenin Antibody (Carboxy-terminal Antigen)
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货号: 9587S 品牌: CST 反应种属:
Human/Mouse/Rat/Monkey
Human/Mouse/Rat/Monkey
应用:
W/IP/IHC-P/IHC-F/ChIP
W/IP/IHC-P/IHC-F/ChIP
规格: 100ul
产品说明书
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价格: ¥ 3600.00

目录价: ¥3600.00

数量
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概述
反应种属
Human/Mouse/Rat/Monkey
来源宿主
Rabbit
应用
W/IP/IHC-P/IHC-F/ChIP
目标/特异性
Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.
使用方法
WB(1:1000) IP (1:50) IHC-P (1:1600) IHC-F (1:400) ChIP (1:25)
是否允许进口
允许
是否危险品
非危险品
性能
供应商
CST
灵敏度
Endogenous
背景

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

存放说明
-20C
计算分子量
92

参考图片

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen) in the presence of control peptide (left) or beta-Catenin blocking peptide #1002 (right).

在对照多肽(左)或beta-Catenin封闭多肽#1002存在的条件下,使用 β-Catenin抗体(羧基末端抗原)对石蜡包埋的人乳腺癌组织进行免疫组化分析。

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对293和NIH/3T3细胞提取物进行western blot分析。

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin Antibody (Carboxy-terminal Antigen).

beta-Catenin对石蜡包埋的人结肠癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对石蜡包埋的人肺癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用beta-Catenin对石蜡包埋的人透明细胞癌组织进行免疫组化分析。

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对石蜡包埋的Apc(Min/+)小鼠肠腺瘤组织进行免疫组化分析。

Immunohistochemical analysis of frozen HCC827 xenograft using beta-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对冰冻的HCC827爪蟾进行免疫组化分析。

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

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