ProDots Recombinant Human Sonic Hedgehog/Shh (C24II) Protein (50 UG)
货号: PRD1845-10 品牌: R&D Systems
规格: 50UG
产品说明书
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价格: ¥ 6721.00

目录价: ¥6721.00

数量
- +
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概述
简介
Supplied as ready-to-use 5 ug protein balls
别名
HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
是否允许进口
允许
是否危险品
非危险品
性能
供应商
R&D Systems
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
内毒素水平
<0.01 EU per 1 μg of the protein by the LAL method.
生物活性
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 0.1-0.4 μg/mL.
溶解方法
For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot
背景
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Accession #
NP_000184
  • N-termi
  • 预期分子量
    20 kDa
    SDS-PAGE
    22 kDa, reducing conditions
    存放说明
    4C
    纯度
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    参考文献
    • Axon Guidance and Neuron Migration
    • EMT Induction
    • Hedgehog Family
    • Hedgehogs
    • Morphogens
    • Retinal Stem Cell Markers

    参考图片

    ProDots® Recombinant
    Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 0.1-0.4 μg/mL.

    1 μg/lane of ProDots® Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R)  and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa.

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