Caspase-1/ICE Fluorometric Assay Kit (100 TESTS)
货号: K110-100 品牌: Biovision 同种亚型:
Rabbit IgG
Rabbit IgG
库存: 公司现货
规格: 100TESTS
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价格: ¥ 6574.00

目录价: ¥6574.00

数量
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概述
描述
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on detection of cleavage of substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). YVAD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
同种亚型
Rabbit IgG
来源宿主
Rabbit
是否允许进口
允许
是否危险品
非危险品
性能
供应商
Biovision
检测类型
Fluorescence (400 nm excitation filter and 505 nm emission filter) • Sample type- Cell and tissue lysates • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 200, 400 assays) • Applications- Detect early/midd
产品特色
• Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
试剂盒组分
• Cell Lysis Buffer • 2X Reaction Buffer • YVAD-AFC (1mM) • DTT (1 M)
存放说明
-20C
原产地
UNITED STATES
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